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Objective: To reduce environmental biohazards, technicians should be educated with safe biological waste disposal procedures and dental material recycling. The present study assesses the retentive force, deformation test and XRD analysis of re-injection acetal resin clasps. Material and Methods: specimens were prepared for clasp retention test injection according to the manufacture's instruction for acetal resin. The first group represent control group with (100% New) and other five groups as experimental groups (group II (25% old +75% new), group III (50% old + 50%) new, and group IV (75% old + 25% new) with different percentages between new acetal materials and old acetal materials, while two last groups with several times (group V (100% old) and group VI (re-injection two times)) only used old acetal materials. The X-ray diffraction (XRD) device used to give structural information for the materials to be tested. The study data were analyzed via One-way ANOVA (LSD) at a significant P-value of (p≤0.05) and a confidence level of 95%. Results: After comparing the results, a significant improvement in the retentive force of acetal clasp specimens after re-injection two times there was found high mean value in group re-injection two times and lower mean value recorded in control group. In XRD patterns of there was no diffraction peak refers to crystal structure of acetal resin. Conclusion: the present study findings concluded that the recycling of acetal resin enhanced the retentive force of acetal clasp and not affected on the crystal structures of material(AU)
Objetivo: Para reduzir os riscos biológicos ambientais, os técnicos devem ser educados com procedimentos seguros de eliminação de resíduos biológicos e reciclagem de material dentário. O presente estudo avalia a força de retenção, teste de deformação e análise de DRX de grampos de resina acetal reinjetada. Material e Métodos: as amostras foram preparadas para o teste de retenção dos grampos e a injeção realizada de acordo com as instruções do fabricante para resina de acetal. O primeiro grupo representa o grupo controle com (100% novo) e os outros cinco grupos como grupos experimentais (grupo II (25% antigo + 75% novo), grupo III (50% antigo + 50% novo) e grupo IV (75% antigo + 25% novo) com porcentagens diferentes entre materiais de acetal novos e antigos, enquanto os dois últimos grupos (grupo V (100% antigo) e grupo VI (reinjeção duas vezes)) utilizaram apenas materiais de acetal antigos. O dispositivo de difração de raios X (DRX) foi utilizado para fornecer informações estruturais dos materiais a testados. Os dados do estudo foram analisados por ANOVA um fator (LSD) com um valor P significativo de (p≤0,05) e um nível de confiança de 95%. Resultados: Após a comparação dos resultados, foi encontrada uma melhora significativa na força de retenção das amostras de grampo de acetal após duas reinjeções, além disso, foi registrado um maior valor médio no grupo reinjeção duas vezes e menor valor médio no grupo controle. Nos padrões de DRX não houve pico de difração referente à estrutura cristalina da resina acetal. Conclusão: os resultados do presente estudo concluíram que a reciclagem da resina de acetal aumentou a força retentiva do grampo e não afetou as estruturas cristalinas do material(AU)
Sujets)
Crochets dentairesRésumé
Medicinal plants are considered colossal producers of bioactive therapeutics agents. Fruit is an essential part of the human diet and is of great interest because of its richness in phytochemicals. The present study was chosen the objectives are assessment the antibacterial activity against the dominantly affected seven Urinary Tract Infected (UTI) pathogenic bacterial species plant leaf extracts and fruits such as Mentha piperita (L.), Flacourtia jangomas (Lour) Raeusch. Experimental UTI pathogenic organisms named as Alcaligens sp., Kliebsiella sp., E. coli, Proteus sp., Staphylococcus sp., Pseudomonas sp., Hence the current result has been clearly depicted that the methanol extract of Mentha piperita leaf expressed the potential signicant antibacterial activity against the ve UTI pathogenic organisms such as Alcaligens sp., and Klebsirlla sp. Proteus species and Pseudomonas sp., than other two experimental extracts of benzene and ethanol. From the present result clearly noticed three elucidated compounds are act as an antibacterial bio-compounds such Octadecanoic acid methyl ester, 1, 2, 4, 5-tetramethyl Piperone and α-Propyl tetradecanol present in methanol leaf.extract of M. piperita (L.) plant. Similarly F. jangomas (Lou.) fruit extract (methanol).possessed the antibacterial property biologically effective compounds named as Lavandulol, α-Humulene and Terpineol. Therefore, both experimental plant leaf and fruit extract possessed signicant antibacterial activity against the tested seven UTI clinical pathogenic organisms Furthermore nanoparticle study also denoted both experimental methanol extracts of M. piperita (L.), (leaf) F.jangomas (Lour) expressed the signicant nanoparticle production. It was noticed the range between 21.50 to 41.03nm and 60.52 to 19.22nm. for M. piperita (L.) F. jangomas fruit juice extract respectively. From the current research clearly showed that methanol extract of both experimental samples could be act as a potential traditional plant based medicines for urinary tract infected diseases.
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ObjectiveTo analyze the polarized light microscopic characteristics， the composition of physical phases and their relative contents of Maifanitum from different origins， and to establish the Fourier characteristic fingerprint of Maifanitum powder crystals by X-ray diffraction（XRD）. MethodA total of 26 batches of Maifanitum samples were selected， and the microscopic characteristics of the sample powders and grinding flakes were observed by polarized light microscopy under single polarized light and orthogonal polarized light， and the main phase compositions and their relative contents were analyzed by powder crystal XRD technique， and the XRD Fourier characteristic fingerprint of Maifanitum was established. The incident light source of XRD was Cu target Kβ radiation， the light tube voltage and light tube current were 40 kV and 40 mA， respectively， the divergence slit was 1°， the scattering slit was 1°， the receiving slit was 0.2 mm， the scanning speed was 5°·min-1 with continuous scanning and scanning range of 5-90°（2θ）， and the step length was 0.02°. ResultThe polarized light micrographs of powders and grinding flakes of Maifanitum were obtained， and the main phases were plagioclase， potassium feldspar and quartz， and a few samples also contained illite， pyrite， iron dolomite， calcite， iron amphibole and chlorite， etc. The relative total content of feldspar phases was 61.9%-82.4%， and the relative content of quartz was 12.6%-33.6%. The XRD Fourier fingerprint analysis method of Maifanitum with 13 common peaks as the characteristic fingerprint information was established， and the similarity calculated by the mean correlation coefficient method was 0.920 9-0.997 7， the similarity calculated by the mean angle cosine method was 0.940 5-0.998 4， the similarity calculated by the median correlation coefficient method was 0.921 1-0.997 5， and the similarity calculated by the median angle cosine method was 0.947 5-0.998 2. ConclusionThe polarized light microscopic identification characteristics of Maifanitum are mainly plagioclase， quartz and potassium feldspar， and the technique of powder crystal XRD Fourier fingerprint analysis can be used for the identification of Maifanitum.
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Synthesis and characterization of halosulphate-based phosphors is important for thermoluminescence dosimetry (TLD), radiophotoluminescence dosimetry (RPL) and scintillator materials. The enhancement of luminescence output in halosulphate-based phosphors and it may be useful for lamp, solid-state lamp and radiation. Dosimetry by activator as well as sensitizer are well known properties. The combustion technique is not applicable for the synthesis of TLD phosphors due to very fine particles, which show less TL intensity, while sol-gel, solid-state diffusion, melt method and precipitation methods are applicable for TLD phosphors. Two halosulphates namely Na21( SO4 ) 7 F6 Cl and 2K3Ca2(SO4)3F were prepared and doped with Dy and Tm for different concentration .Halosulphate , Na21( SO4 ) 7 F6 Cl was prepared by wet chemical method and Halosulphate , 2K3Ca2(SO4)3F was prepared by solid state diffusion method . The characterization was done by X - ray diffraction ( XRD ) , Thermo luminescence (TL) was also studied . For Dy doped Na21( SO4) 7 F6 Cl , The peak was observed at 1200 C and shoulder at 1750C for 0.2 % molar concentration of Dy. and for 2K3Ca2(SO4)3F doped with Tm the shoulder peak was observed at 240 0 C and at 150 0C for 0.7 % molar concentration of Tm.
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In the current scenario, microbial infections are major concern and they multiply so rapidly throughout the world; also, they cause serious bio illness and can even results in death among humans. Complexes framed by crystalline metal ions have been used as medications, because of a wide range of organic activities against harmful microorganisms. In the present study, greenish blue potassium tetrachlorocuprate (II) dihydrate K2CuCl4.2H2O crystalline precipitates were prepared by solvent evaporation method at ambient temperature. This complex was found in nature as a rare mineral Mitscherlichite. For finding the crystalline parameters of the sample, a Single-crystal XRD analysis was used and the data confirms. K2CuCl4.2H2O crystallizes into a tetragonal structure. The formation of bonds and the presence of functional groups in the complex were determined from Surface morphology studies, the very clear morphology of the complex has smoothing surface, defect-free, small microstructures and stacklike shapes of a well-defined crystalline pattern. Also, addition, optical analysis supports that its possibility in antimicrobial applications. The sample shows comparatively good results in positive control of antibacterial and antifungal activities, namely Gentamicin, Chloramphenicol and Clotrimazole. IC50 and optical density (OD) values were used to determine the cytotoxicity and cell viability, respectively. In vitro cytotoxicity of MTT assay shows in graphical abstract.
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Suryaprabha gulika is a Kharaleeya preparation mentioned in the Gulika prakarana of Sahasrayogam indicated for Sula, Swasa, Kasa and Mahajwara. It contains eleven ingredients namely Suddha Parada, Suddha Gandhaka, Suddha Hingu, Amalaki, Harithaki, Vibheethaki, Sunti, Pippali, Maricha, Yavani and Suddha Vatsanabha triturated for 6 hours in Jambeera swarasa. In this paper, the work done on pharmaceutical and analytical aspects of Suryaprabha gulika is detailed. Pharmaceutical study included Sodhana of Parada, Sodhana of Gandhaka, preparation of Kajjali, Sodhana of Hingu, Sodhana of Vatsanabha and the preparation of final product whereas the analytical study included evaluation of organoleptic characters, physico chemical parameters and analysis based on modern instrumentation techniques such as X-ray Fluorescence (XRF), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and particle size analysis.
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Background & objectives: Mosquitoes are insects of public health importance that act as a vector to transmit various vector-borne diseases in humans including dengue, malaria, filariasis and yellow fever. The continually employed synthetic insecticides have developed resistance in mosquitoes. Nano-based botanical insecticides can be considered as the best alternative due to several advantages like being simple, non-pathogenic, biodegradable and safe to the environment. The present work reported the maximum larvicidal potential of green synthesized silver nanoparticles (AgNPs) derived from the leaf extract of Solanum xanthoearpum against the third instar larvae of Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus over its crude leaf extract. Methods: The synthesis of AgNPs was done by adding leaf extract into silver nitrate solution in a conical flask. The characterization of AgNPs was done using different techniques such as UV-Vis, SEM, TEM, XRD, DLS and SAED. FT-IR analysis was done to find out the compound responsible for bio-reduction of silver nitrate. Larvicidal activity of AgNPs was checked against An. stephensi, Ae. aegypti, and Cx. quinquefasciatus according to WHO standard protocol and toxicity was evaluated against Poecilia reticulate. Results: A change in colour was observed indicating the synthesis of AgNPs which was further confirmed by a strong surface plasmon resonance peak at 421nm under the UV-Vis spectrum. SEM and TEM micrographs exhibited that the most common shape of AgNPs was spherical. XRD spectrum showed crystalline nature of silver nanoparticles. FT-IR spectrum showed the presence of various functional groups such as carboxyl and hydroxyl which might be responsible for bio-reduction and capping of silver nanoparticles. Further, silver nanoparticles were very effective against An. stephensi, Ae. aegypti, and Cx. quinquefasciatus with LC50 and LC90 values of 1.90, 2.36, 2.93, 3.82, 4.31 and 7.63 ppm, respectively, as compared to aqueous leaf extract after 72 h of exposure and were non-toxic against non-target organism P. retieulata. Interpretation & eonelusion: From the above finding, it can be concluded that fabricated AgNPs can be promising eco-friendly tools for controlling mosquito vectors.
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The title Zn (II) complex was synthesized by reacting the compound Bis-[(E)-3{2-(1-4-chlorophenyl) ethylidiene}hydrazinyl]-N-(4-methylphenyl)-3-oxo propanamide with Zn (II) chloride dihydrate in alkaline dimethylsulphoxide and ethanol solution under reflexing condition for 8 h. The resultant compound was filtered and recrystallized from ethanol. The hydrazone Schiff base ligand and its Zn (II) complex were characterized by using UV-Vis spectroscopy and XRD, TEM and SEM analysis. The antibacterial activities of ligand and its Zn complex were examined using disc diffusion method. The spectra results showed that the hydrazone ligand undergoes keto-enoltautomerism forming a bidentated ligand (N,N) towards Zn+2 (II) ion. It is very interesting that on sides of the two hydrazone ligands which coordinate to the Zn+2 ions, an additional two thiosemicarbazine moiety were also coordinated with Zn+2 ions in the crystalline powder, resulting in a hexa coordinated octahedral Zn (II) complex. Both hydrazone Schiff base ligand and its Zn (II) complex were found to exhibit good antibacterial activity even when the concentrations were high. Molecular docking analysis also deciphered that Zinc complex and carbohydrazone ligand both were found to be fitted into the active sites of molecular targets and Zn complex showed better binding affinities towards macromolecules compared to ligand.
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Abstract The goal of the present study was to develop inclusion complexes and polymers dispersions of ramipril prepared by physical mixing, kneading, co-evaporation, and solvent evaporation methods to enhance drug solubility and dissolution rate, and thereby to reduce drug dose and side effects using selected hydrophilic carriers such as β-CD, PVP-K25, PEG 4000, and HPMC K100M. The prepared formulations were characterized for solubility and in-vitro drug release studies. The systematic optimization of formulations was performed using I-Optimal experimental design by selecting factors such as type of carriers (X1), drug: carrier ratio (X2), and method of preparation (X3), and response variables including percent yield (Y1), solubility (Y2), Carr's index (Y3) and drug release in 30 min (Y4). Mathematical modeling was carried out using a quadratic polynomial model. The inclusion complex formulation (F27) was selected as an optimized batch by numerical desirability function and graphical optimization with the help of design space. The inclusion complex prepared by the co-evaporation method showed maximum drug solubility and released in pH 6.8 phosphate buffer compared to pure and other formulations. The inclusion complex is a feasible approach to improve the solubility, dissolution rate, bioavailability, and minimization of drugs' gastrointestinal toxicity upon oral administration of ramipril.
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Introducción: Alternaria spp y Candida spp. son hongos patógenos de ambientes interiores como la casa, la oficina, el aula, etc., causan enfermedades como asma crónica e infecciones sistémicas en individuos inmunodeprimidos a través de la secreción de diversas sustancias tóxicas. Los ambientadores a base de productos químicos disponibles comercialmente que se utilizan para controlar la carga de hongos en el ambiente interior no son beneficiosos para la salud humana. Objetivo: proporcionar una alternativa viable en forma de enfoque basado en nanopartículas para el manejo de hongos transmitidos por el aire. Metodología: aislamiento, identificación microscópica y bioquímica de hongos de interior; Síntesis de nanopartículas de azufre (SNP) mediadas por Azadirachta indica, su detección y caracterización; y evaluación in vitro de SNP contra hongos aislados presentes en el ambiente interior. Resultado: Los hongos aislados se identificaron como especies de Alternaria spp y Candida spp. Los SNP mostraron máximos de absorbancia a 291 nm. El análisis NTA mostró un tamaño medio de 188,4 nm y un potencial zeta de -4,94 mV, lo que representa una síntesis de SNP estables. El patrón XRD confirmó la naturaleza cristalina cúbica centrada en la cara de los SNP. El espectro FTIR representó la presencia de compuestos polihidroxilo, nitrilo, ceto, aromáticos y carboxílicos que estabilizaron los SNP. Los ensayos antifúngicos demostraron la actividad significativa de los SNP formulados y del ambientador infundido con aceite de eucalipto. Conclusión: Los SNP mediados por A. indica se pueden aplicar en la formulación y fabricación de un ambientador ecológico para el manejo de hongos patógenos de interior como Alternaria spp y Candida spp.
Introduction: Alternaria spp. and Candida spp. are the main fungal pathogen of indoor environment like house, office, classroom, etc. These may cause various diseases and infections like systemic infections, or chronic asthma in immunocompromised individuals through secretion of various toxic substances. Chemical-based commercially available room fresheners used to control the fungal load of indoor environment are not beneficial to human health. Objective: was to provide viable alternative in the form of nanoparticle-based approach for the management of air-borne fungi. Methodology: The present study primarily focuses on the isolation, microscopic and biochemical identification of indoor fungi; Azadirachta indica-mediated sulphur nanoparticles (SNPs) synthesis, their detection and characterization; and in vitro assessment of SNPs against isolated fungi present in indoor environment. Result: The isolated fungi were identified as Alternaria spp and Candida spp. The SNPs showed absorbance maxima at 291 nm. NTA analysis showed average size of 188.4 nm, and zeta potential of -4.94 mV which represented synthesis of stable SNPs. XRD pattern confirmed the face centered cubic, crystalline nature of SNPs. FTIR spectrum depicted the presence of polyhydroxyl, nitrile, keto, aromatic and carboxylic compounds which stabilized the SNPs. The antifungal assays demonstrated the significant activity of the formulated SNPs and eucalyptus oil infused air freshener. Conclusion: It can be concluded that A. indica-mediated SNPs can be applied in the formulation and manufacture of an ecofriendly air freshener for the management of indoor fungal pathogens like Alternaria spp. and Candida spp.
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Nanoparticules , Antifongiques , Candida , AlternariaRésumé
Background: Bhasmas are traditional Ayurvedic medicines prepared from minerals and metals by tediousprocess which removes toxic properties of metals and minerals and enhances medicinal properties. Wehave synthesized abhraka bhasma by two traditional methods and were analyzed during each stage ofpreparation.Objective: The present study deals with the synthesis and characterization of abhraka bhasma by twodifferent methods, method-1 is commonly used for treating anemia and tropical sprue and method-2 fortreating chronic cough. Hence attempts have been made to see the physico-chemical differences between these methods by using different analytical techniques.Material and methods: Different steps in preparation of abhraka bhasma includes shodhana (purification),dhanyabhraka, marana (incineration) and amritikarana. The prepared bhasma were analyzed by classicalayurvedic tests and modern analytical techniques like XRD, FTIR, Raman spectroscopy, SEM, TEM, EDX,BET, DLS and TGA/DTA.Results: The morphological characterization revealed that as-prepared abhraka bhasma products werenano crystalline in nature. Elemental analysis confirms presence of various elements along with carbonsuggesting bhasma as herbo-mineral compound. The XRD studies revealed the presence of KMg3(Si3Al)O10(OH)2 in bhasmas.Conclusion: Characterization study revealed presence of various bonds of different functional groups andformation of nano particles. Abhraka bhasma prepared by method-2 has more proportion of nano particles than that prepared by method-1 and hence will be more effective in its use.
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Background: Yashada bhasma has been found to be very useful for the treatment of ailments like diabetes, eye disorder, urinary disorder etc. Since bhasma is a metallic preparation, so to prove its nontoxicity; modern standardization of the prepared samples is a must apart from other organoleptictests as mentioned in the ancient text.Objectives: The present study is aimed to synthesize bio-compatible Yashada bhasma from bioincompatible zinc metal. Further, comparative study of their chemical and physical properties throughsome quality control tests, physico-chemical tests and modern tests like XRD, DLS, Zeta potential, SEMand EDAX are carried out.Materials and methods: Yashada bhasma is prepared by a three step process i.e. Shodhan, Jarana andMarana. The inclusion of plant extracts and herbs during calcination process enhances its medicinalqualities, and reduces it to a nano size.Results: The XRD analysis of Yashada bhasma shows hexagonal ZnO crystalline phase whereas the rawmetal confirms the presence of crystallite Zinc metal. DLS shows reduction in particle size of YashadaBhasma (339.8 nm) as compared to raw metal (2063 nm) and this reduction is further supported by SEMwhich shows the particle size of Yashada bhasma (324 nm) and raw metal (1-2m). The zeta potential valueconfirms the stability of Yashada bhasma. EDAX revealed difference in concentration of Zinc and Oxygenin both the samples.Conclusion: An effort has been made to characterize the preparation of Yashada bhasma using sophisticated analytical tools as a step towards standardization of the bhasma. The results help in scientificallyestablishing the findings in line with the principle of Ayurveda.
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Objective: The present goals of our study were biological synthesis, characterizations of silver nanoparticles, and evaluation of its antimicrobial activity against microbial pathogens like Escherichia coli, Enterococcus faecalis, Streptococcus pneumoniae and Staphylococcus aureus. Methods: The bacterial Strain NS-24 was isolated on nutrient agar medium and was selected for the synthesis of silver nanoparticles based on its gram-negative characteristics. The characterizations of silver nanoparticles were done by UV-Visible spectroscopy, Atomic Force Microscopy (AFM), High Resolution-Transmission Electron Microscopy (HR-TEM), Scanning Electron Microscopy (SEM) with Energy Dispersive Spectroscopy (EDX), X-ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). Later, the molecular characterization of the Strain NS-24 was done by DNA extraction and 16S rRNA gene sequencing. Results: The UV-visible spectrophotometric observation of the Strain NS-24 supernatant and AgNO3 solution showed maximum absorbance at 423 nm. The AFM data confirmed that the particles were polydispersed and spherical in shape. Additionally, the FTIR analysis revealed the IR spectral band patterning and TEM analyzes showed the size of biological AgNPs was in the range of 12.56 nm to 27.32 nm, with an average of 18.06 nm in size. Further, the 16S rRNA gene sequencing revealed the identity of Strain NS-24 as Stenotrophomonas maltophilia. The antimicrobial activity of AgNPs was studied on different gram-negative and gram-positive bacterial strains like Escherichia coli (MTCC 40), Enterococcus faecalis (MTCC 6845), Streptococcus pneumoniae (MTCC 8874) and Staphylococcus aureus (MTCC 2825), which showed good inhibition of their growth at varying concentrations of AgNPs against all the pathogens. Conclusion: Our findings showed that the synthesized AgNPs from the isolated bacterium was small in size and had profound antibacterial activity against pathogenic micro-organisms.
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Objective: To formulate silver nanocomposites from Achyranthes aspera leaf extracts and evaluate its larvicidal activity against Aedes aegypti. Methods: The silver nanocomposites were synthesized from Achyranthes aspera leaf extracts. The process was optimized and traced through UV-visible and photon correlation spectroscopy. The larvicidal potential of silver nanocomposites of Achyranthes aspera leaf extracts was assessed against the early fourth instars of Aedes aegypti and three non-target organisms. Furthermore, the most effective and eco-safe nanocomposite was characterized by different biophysical techniques including scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform-infrared spectroscopy (FT-IR). Results: The formulated silver nanocomposites exhibited efficient larvicidal efficacy against Aedes aegypti. Bioassay with silver nanocomposites formulated using different AgNO 3 concentrations (3, 4, and 5 mM) revealed respective LC
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Objective: To formulate silver nanocomposites from Achyranthes aspera leaf extracts and evaluate its larvicidal activity against Aedes aegypti.Methods: The silver nanocomposites were synthesized from Achyranthes aspera leaf extracts. The process was optimized and traced through UV-visible and photon correlation spectroscopy. The larvicidal potential of silver nanocomposites of Achyranthes aspera leaf extracts was assessed against the early fourth instars of Aedes aegypti and three non-target organisms. Furthermore, the most effective and eco-safe nanocomposite was characterized by different biophysical techniques including scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform-infrared spectroscopy (FT-IR). Results: The formulated silver nanocomposites exhibited efficient larvicidal efficacy against Aedes aegypti. Bioassay with silver nanocomposites formulated using different AgNO3 concentrations (3, 4, and 5 mM) revealed respective LC50 values of 37.570, 6.262 and 1.041 μg/mL; 5.819, 1.412 and 0.489 μg/mL; and 5.519, 1.302 and 0.267 μg/mL after 24, 48 and 72 h. The silver nanocomposites with 4 mM AgNO3 were selected for characterization. SEM and TEM analysis revealed spherical, poly-dispersed structure with varied diameters of 1-25 nm. The XRD analysis established the crystalline and face-centred-cubic structure of silver nanocomposites with the maximum peak at a 2θ value of 37.42°. The EDX pattern showed the presence of Ag, O and C in the nanocomposites in their order of weight％. The FT-IR displayed visibly distinct peaks in different ranges demonstrating the intricacy of silver nanocomposites. In addition, the lethal concentrations of silver nanocomposites of Achyranthes aspera leaf extracts against Aedes aegypti larvae were non-toxic to non-target organisms including Gambusia affinis, Daphnia magna and Moina macrocopa. Conclusions: Silver nanocomposites synthesized with leaf extract of Achyranthes aspera provide a cost-effective and eco-safe alternative to conventional insecticides, and can be utilized as a potent mosquito nano-larvicide.
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The present investigation, the successful preparation of pure ZnO (Z1) NPs and SrBa dual doped ZnO (Z2) NPs by chemical co-precipitation technique without use of any capping agent. The structural and morphological properties of Z1 and Z2 NPs were analyzed using X-ray diffraction (XRD) studies, Field emission scanning electron microscopy (FESEM), Elemental analysis (EDAX), Fourier transform infrared spectroscopy (FTIR). An optical property was studied by UV–Vis spectroscopy and Photoluminescence (PL) spectra. The antimicrobial activity of Z1 and Z2 NPs has been investigated against Staphylococcus aureus and Klebsiella pneumoniae bacterial strains. It has been interestingly observed that Z2 NPs has enhanced the inhibitory activity than that of Z1 NPs against S. aureus and more efficiently than the K. pneumoniae bacterial strain.
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Introduction: The biological green synthesis of nanoparticles via nanobiotechnology processes have a significant potential to boost nanoparticles production without the use of harsh, toxic, and expensive chemicals commonly used in conventional physical and chemical processes. Annona muricata, a tropical plant belonging to family Annonaceae is one of the most used plants in folk medicine because of its many medicinal uses and therefore presents a strong candidate for use in green synthesis. Aims: The aim of this study was to optimize a method for the synthesis of Silver Nanoparticles (AgNPs) from ethanolic extracts of leaves of Annona muricata as well as to characterize the green synthesized AgNPs. Methodology: AgNPs were synthesized from Annona muricata leaves using AgNO3 solution. The AgNPs were characterized using spectroscopy and microscopy techniques. Results: The formed AgNPs had an absorption maximum at 429 nm using UV–Visible spectroscopy and were stable under different pH, temperature, and storage conditions. Fourier transform infrared analysis revealed the different functional groups responsible for the synthesis and stabilization of the AgNPs. Scanning electron microscopy analysis revealed a spherical nature of the synthesized AgNPs. Energy dispersive x-ray spectroscopy analysis showed presence of Ag, O, and Cl with Ag having the highest composition at 60%. X-Ray Diffraction and Dynamic Light Scattering revealed a crystalline nature of AgNPs with an average size of 87.36 nm and a polydispersity index of 0.16 respectively. Transmission Electron Microscopy analysis further confirmed the crystalline and spherical nature of the AgNPs. Conclusion: In this article, an efficient, eco-friendly and low-cost method for the synthesis and recovery of stable AgNPs using Annona muricata leaves ethanolic extracts as both a reducing and capping agent has been reported for the first time. The synthesized AgNPs could be promising candidates for many biomedical, clinical, engineering, and polymer applications.
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Rasamanikya prepared from Shodhita Haritala is one of the effective and economical medicines used in different skin and respiratory disorders. Different methods are explained in the classics for the preparation of Rasamanikya and few adopted methods are also developed from scholars of Rasashastra depending upon their experience. Present study is aimed at exploring all these methods in detail and any modifications if needed. The Patra Haritala is subjected to different Shodhana procedures in different media, the changes observed are discussed in the article. The yield of Shodhita Haritala was 92% to 96% in different Shodhana methods. Rasmanikya is prepared in six different methods. Sharava samputa method (Method III & IV) can be considered as suitable methods for pharmaceutical preparation of Rasamanikya in large quantity as there was loss of only 11% to 13% drug was noticed. Chemical analysis and X-Ray diffraction of Patra Haritala and Rasamanikya prepared from all the methods is carried as a part of standardization. In Analytical study of all the methods, 44% to 47% of Arsenic and 22 to29% of Sulphur was present in Rasamanikya. X-RD study of Haritala and Rasamanikya samples revealed that crystalline form of Haritala was changed to relatively amorphous form in Rasamanikya prepared by I, II, III and IV methods, which indicates quick and better absorption of the drug Rasamanikya on administration making it one of the economical and potent medicine. Chemically Rasamanikya can be considered as a complex compound of As2S3 and As2O5.
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Objective: Ammonium alum is a common counterfeit of Alumen,and the processed product of ammonium alum is a common counterfeits of calcined Alumen. This paper aims to establish a method for identifying Alumen,calcined Alumen,ammonium alum and their processed products. Method: The samples were analyzed by scanning electron microscope (SEM) and X ray diffraction (XRD) in this paper. Result: Ammonium alum and Alumen showed obvious changes in morphology after processing. Both Alumen and ammonium alum showed obvious differences in morphology at×250 and×1 000 times microscope. Alumen presented irregular fragments,clear edge corners,smooth surface,scattered irregular small particles,occasional holes and longitudinal edges. Ammonium alum presented irregular clumps,blunt edges,not obvious edges and corners,uneven surface,scattered smaller and round-like particles. The difference in morphology was not obvious at×250 times microscope between Alumen and ammonium alum processed products. While at×1 000 times,the surface of calcined Alumen was uneven with coarse particles; the surface of counterfeit calcined Alumen was flat,and the coarse particle characteristics were not obvious. XRD can be used to rapidly and accurately identify the primary phase of Alumen,calcined Alumen,ammonium alum and ammonium alum processed products:KAl(SO4)2·12H2O,NH4Al(SO4)2·12H2O,KAl(SO4)2,and NH4Al(SO4)2 respectively, with 2θ angle characteristic value of 23,12,22 and 5 respectively for XRD peak. Conclusion: SEM and XRD techniques can be used for the identification of Alumen,calcined Alumen,ammonium alum and their counterfeit products.
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PURPOSE: The purpose of this in vitro study was to evaluate the effect of surface grinding and polishing procedures using high speed zirconia diamond burs with different grit sizes on the phase transformation and flexural strength of zirconia.